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Merck

53567-U

Supelco

Ascentis® Express F5, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

材料

stainless steel column

品質等級

agency

suitable for USP L43

產品線

Ascentis®

特點

endcapped

製造商/商標名

Ascentis®

包裝

1 ea of

參數

60 °C temp. range
600 bar max. pressure (9000 psi)

技術

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

長度 × 內徑

5 cm × 2.1 mm

表面積

135 m2/g

雜質

<5 ppm metals

基質

Fused-Core particle platform
superficially porous particle

基質活性組

PFP (pentafluorophenyl) phase

粒徑

2.7 μm

孔徑

90 Å pore size

工作pH值

2-8

應用

food and beverages

分離技術

reversed phase

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一般說明

由于存在电负性氟,Ascentis Express F5 的五氟苯基丙基固定相可提供具有缺电子苯环的稳定反相填料。除了形成 pi-pi 和温和的空间相互作用外,F5 相也通过极性相互作用保留化合物。Ascentis Express F5 可用于碱性、酸性或中性化合物,选择性与 C18 不同。

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Lot/Batch Number

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

商品

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

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实验方案

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

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