生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3H17, recombinant monoclonal
monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 38.39 kDa
observed mol wt ~58 kDa
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... DKK3(27122)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 3H17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Dickkopf-related protein 3 (DKK3).
免疫原
His-tagged recombinant fragment corresponding to 300 amino acids (almost full length mature protein) from human Dickkopf-related protein 3 (DKK3).
應用
Affinity Binding Assay: A representative lot of this antibody bound recombinant DKK3 protein with a KD of 5.7 x 10-9 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in MCF-7 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected DKK3 in one million MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in MCF-7 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected DKK3 in one million MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-DKK3, clone 3H17 ZooMAb, Cat. No. ZRB1074, is a recombinant Rabbit monoclonal antibody that targets DKK3 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
標靶描述
Dickkopf-related protein 3 (UniProt: Q9UBP4; also known as Dickkopf-3, Dkk-3, hDkk-3) is encoded by the DKK3 (also known as REIC, UNQ258/PRO295) gene (Gene ID: 27122) in human. DKK is a family of secreted glycoproteins that display Wnt inhibitory activity. They act as high affinity ligands for the transmembrane proteins Kremen1 and 2, which are known to modulate Wnt signaling. They play a role in vertebrate development via locally inhibiting Wnt-regulated processes such as antero posterior axial patterning, limb development, somitogenesis and eye formation. DKK3 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. High expression of DKK3 is reported in heart, brain, and spinal cord. DKK3 is shown to antagonize canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with Kremen protein, which is known to promote internalization of LRP5/6. The C-terminal cysteine-rich domain of DKK3 mediates its interaction with LRP5 and LRP6. DKK3 is shown to induce a redistribution of β-catenin to the cell membrane where β-catenin promotes cell-cell adhesion by binding to the cytoplasmic domain of E-cadherin to form adheren junction complex. This binding also prevents nuclear translocation of β-catenin that reduces Wnt-mediated LEF/TCF transactivation. DKK3 is proposed to act as a tumor suppressor protein and its over-expression is shown to suppress tumor growth. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hoang, BH., et al. (2004). Cancer Res. 64(8); 2734-2339; Niehrs. C. (2006). Oncogene. 25(57); 7469-7481).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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