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Merck
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Key Documents

SRP2018

Sigma-Aldrich

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

别名:

E1A

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.26

生物源

Saccharomyces cerevisiae
canine

重組細胞

expressed in E. coli

化驗

≥70% (SDS-PAGE)

形狀

frozen liquid

分子量

~33 kDa

包裝

pkg of 4 μg

儲存條件

avoid repeated freeze/thaw cycles

濃度

100 μg/mL

技術

electrophoretic mobility shift assay: suitable

顏色

colorless to clear

NCBI登錄號

J04368
NM_001184062

UniProt登錄號

P04386

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

Saccharomyces cerevisiae ... GAL4(855828)

生化／生理作用

The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.

外觀

Clear and colorless frozen liquid solution

準備報告

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
A J Berk et al.
Cell, 17(4), 935-944 (1979-08-01)
L Keegan et al.
Science (New York, N.Y.), 231(4739), 699-704 (1986-02-14)
The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription.
T Kodadek
Cellular & molecular biology research, 39(4), 355-360 (1993-01-01)
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5'-CGGN5(T/A)N5CCG-3'. We review the general requirements that must be met for

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