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一般說明
来自酵母的6-磷酸葡萄糖酸脱氢酶包含NADP +结合结构域、α螺旋疏水相互作用结构域和C-末端结构域。它以二聚体的形式存在,C末端区域对于底物的结合和释放至关重要。
應用
来自酵母的6-磷酸葡萄糖酸脱氢酶已被用于确定细胞裂解物中的己糖激酶(HK)和木糖还原酶(XR)活性。
生化/生理作用
通过测量NAD +或NADP +的减少来确定酶活性。据信高水平的NADPH会抑制该酶,而6-磷酸葡萄糖酸酯则可激活该酶。
單位定義
在存在NADP+的条件下,在37°C下,每分钟1个单位将1.0μmol的6-磷酸-D葡萄糖酸氧化为D-核糖5-磷酸和CO2。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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