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Merck

GW20071F

Sigma-Aldrich

Anti-β-Galactosidase antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

别名:

Anti-Beta-Galactosidase

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

chicken

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

bacteria

製造商/商標名

Genway 15-288-20071F

技術

western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

免疫原

Immunogen Sequence: GI # 26246355, sequence 1-1024
Recombinant β-galactosidase [Escherichia coli CFT073]

應用

Anti-β-Galactosidase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

生化/生理作用

The lac operon (lactose operon) is an essential operon used for the transport and metabolism of lactose in Escherichia coli and some enteric bacteria. It has three adjacent structural genes, lacZ, lacY and lacA. The lacZ gene encodes β-galactosidase. Escherichia coli β-galactosidase is mostly used as a reporter enzyme. It is produced under oxidizing conditions in the cytoplasm of an Origami strain. β-galactosidase occurs in a disulfide bond. At the active site of β-galactosidase a loop opens and closes during catalysis.

外觀

Solution in phosphate buffered saline containing 0.02% sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Rebecca E Harvey et al.
Endocrinology, 158(7), 2179-2189 (2017-04-04)
Ghrelin plays a key role in appetite, energy homeostasis, and glucose regulation. Recent evidence suggests ghrelin suppresses inflammation in obesity; however, whether this is modulated by the acylated and/or des-acylated peptide is unclear. We used mice deficient in acylated ghrelin
Joaquin Seras-Franzoso et al.
Applied and environmental microbiology, 78(7), 2376-2385 (2012-01-31)
Escherichia coli β-galactosidase is probably the most widely used reporter enzyme in molecular biology, cell biology, and biotechnology because of the easy detection of its activity. Its large size and tetrameric structure make this bacterial protein an interesting model for
Larisa J Jancewicz et al.
Archives of biochemistry and biophysics, 517(2), 111-122 (2011-12-14)
A loop (residues 794-803) at the active site of β-galactosidase (Escherichia coli) opens and closes during catalysis. The α and β carbons of Ser-796 form a hydrophobic connection to Phe-601 when the loop is closed while a connection via two

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