推荐产品
ligand
recombinant protein A (E. coli)
儲存期限
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包裝
pack of 200 mL
製造商／商標名
Cytiva 17-1279-03
儲存條件
(20% Ehtanol)
基質
4% cross-linked agarose
粒徑
60-165 μm
平均直徑
90 μm (d50v)
cleaning in place
2-11
工作範圍
3-10
容量
~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
相关类别
一般說明
rProtein A Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.
rProtein A Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, rProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特點和優勢
- High dynamic binding capacity due to oriented, single-point attachment of protein A
- Well-established Protein A medium used in many approved MAb processes
- No mammalian components involved during the manufacturing process
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
实验方案
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
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