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Key Documents

GE17-0444-01

Lentil Lectin-Sepharose 4B 25 mL

Cytiva 17-0444-01

别名:

Sepharose 4B

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

lentil lectin

包裝

pkg of 25 mL

製造商/商標名

Cytiva 17-0444-01

儲存條件

(20% Ehtanol, PBS pH 7)

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

3-12

工作範圍

3-10

儲存溫度

2-8°C

一般說明

Lentil Lectin Sepharose media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

特點和優勢

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

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