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一般說明
来自勃艮第蜗牛的β-葡萄糖醛酸苷酶H-2型是一种源自罗马蜗牛的酶的粗溶液。许多衍生自软体动物的β-葡萄糖醛酸苷酶也具有硫酸酯酶活性。
應用
β-葡萄糖醛酸苷酶可用于通过尿液技术进行的芳香化测定,适用于对芳香化酶抑制剂进行体内评估 。
来自勃艮第蜗牛的β-葡萄糖醛酸苷酶已被用于:
- 在尿液和豆浆样品中,将异黄酮结合物转化为苷元形式,从而用于高效液相色谱(HPLC)检测
- 用作原生质体溶液中的成分
- 用于从血浆和尿液样品中生成芳香族和酚类化合物,从而用于气相色谱-质谱(GC-MS)
- 水解粪便样品中的葡萄糖醛酸苷和硫酸盐共轭代谢物
品質
来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
單位定義
在pH 5.0、温度37°C条件下,一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞(30分钟实验)。
硫酸酯酶单元定义:一单位的硫酸酯酶每小时,pH5.0,37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
外觀
在~1.0 M硫酸铵中含有3 mM叠氮化钠作为保存剂的水相溶液。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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