KARPAS-929

06072606, from human bone marrow

• 别名: KARPAS 929, KARPAS, 929, KARPAS929
• 分類程式碼代碼: 41106514

属性

• 生物源: human bone marrow
• 增長模式: Suspension
• 染色體組型: Hyperdiploid with complex aberrant chromosomes
• 形態學: Round to polygonal cells growing in clusters in suspension
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice

说明

細胞系來源

Human multiple myeloma

細胞系描述

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

DNA分析

Not specified

培養基

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

例行更新培養

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.