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Key Documents

104-05A

Sigma-Aldrich

Human Epidermal Melanocytes: HEM, adult

别名:

HEM cells

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.81

生物源

human skin (normal abdominal)

品質等級

包裝

pkg of 500,000 cells

製造商/商標名

Cell Applications, Inc

增長模式

Adherent

染色體組型

2n = 46

形態學

Epidermal

技術

cell culture | mammalian: suitable

相關疾病

metabolic disorders

運輸包裝

dry ice

儲存溫度

−196°C

一般說明

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.

細胞系來源

Skin

應用

melanin production, melanocyte proliferation, differentiation

成分

Basal Medium containing 30% FBS & 10% DMSO

準備報告

  • 2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO
  • Can be cultured at least 12 doublings

例行更新培養

Please refer to the HEM Culture Protocol.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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