MABN2593

Anti-P2RY12 Antibody, clone 10B5.3.27

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 300
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 10B5.3.27, monoclonal
• 分子量: calculated mol wt 39.44 kDa; observed mol wt ~55 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG2bκ
• 表位序列: Unknown
• Protein ID登錄號: NP_073625.1
• UniProt登錄號: Q9H244
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... P2RY12(64805)

说明

一般說明

P2Y purinoceptor 12 (UniProt: Q9H244; also known as P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999) is encoded by the P2RY12 (also known as HORK3) gene (Gene ID: 64805) in human. P2Y12 receptor is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. Most of its transmembrane domains appear to be tilted and/or kinked. Binding of agonists to P2Y12 receptor promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. It serves as a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. It is not activated by UDP and UTP. It is highly expressed in platelets and lower level of expression levels is observed in the brain, lung, and adrenal glands. It is required for normal platelet aggregation and blood coagulation. It is the predominant receptor involved in the ADP-stimulated activation of the glycoprotein IIb/IIIa receptor. Activation of the glycoprotein IIb/IIIa receptor results in enhanced platelet degranulation and thromboxane production, and prolonged platelet aggregation. P2Y12 receptor has also been reported to be expressed on microglia and is required to neuropathic pain following peripheral nerve injury. Mutations in P2RY12 gene have been linked to bleeding disorders resulting from severe impairment of platelet response to ADP resulting in defective platelet aggregation. (Ref.: Burnstock G. (2009). Curr. Pharm. Des. 15(15);1717-35; Damman, P., et al. (2012). J. Thromb. Thrombolysis. 33(2); 143-153).

特異性

Clone 10B5.3.27 is a mouse monoclonal antibody that detects P2Y purinoreceptor 12. It targets an epitope within 40 amino acids from the C-terminal, cytoplasmic domain.

免疫原

GST-tagged recombinant fragment corresponding to 40 amino acids from the C-terminal, cytoplasmic domain of human P2Y purinoreceptor 12.

應用

Quality Control Testing

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected P2RY12 in human brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected P2RY12 in U251 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected P2RY12 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-P2RY12, clone 10B5.3.27, Cat. No. MABN2593, is a mouse monoclonal antibody that detects P2Y purinoceptor 12 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable