MABF2778

Anti-IRF-3 Antibody, clone AR-1

• 分類程式碼代碼: 12352203

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: AR-1, monoclonal
• 分子量: calculated mol wt 47.22 kDa; observed mol wt ~51 kDa
• 物種活性: rhesus macaque, human
• 包裝: antibody small pack of 100 μL
• 技術: ELISA: suitable; flow cytometry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG1
• UniProt登錄號: Q14653
• 運輸包裝: dry ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

说明

一般說明

Interferon regulatory factor 3 (UniProt: Q14653; also known as IRF-3) is encoded by the IRF3 gene (Gene ID: 3661) in human. IRF-3 is a transcription factor that is constitutively expressed in a variety of tissues and plays a role in intracellular immune response against DNA and RNA viruses. It regulates the transcription of type I interferon genes (IFN-a and IFN-b) and interferon-stimulated genes by binding to an interferon-stimulated response element in their promoters. IRF-3 contains a DNA-binding domain (aa 5-111), nuclear export signal (aa 139-149), IRF-interacting domain, and a C-terminal serine-rich region that contains several phosphorylation sites. Although some of these serines are shown to be phosphorylated in the resting state, further phosphorylation of specific residues is required for its activation and nuclear translocation. In uninfected cells, IRF-3 is present in the cytoplasm, however, following infection, it undergoes phosphorylation on serine 385, serine 386, threonine 390, and serine 396. These phosphorylation events induce a conformational change that leads to its dimerization and nuclear localization where it associates with CREB binding protein to form dsRNA activated factor 1 that subsequently leads to the activation of type I interferons and ISG genes. Phosphorylation and subsequent activation of IRF-3 shown to be inhibited by vaccinia virus protein E3. Mutations in IRF3 gene have been linked to infection-induced, Herpes-specific acute encephalopathy that is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Clone AR-1 recognizes an epitope within the DNA-binding domain of IRF-3. It can detect resting IRF-3 as well as the activated form. (Ref.: Rustagi, A., et al. (2013). Methods. 59(2); 225-232; Honda, K., et al. (2006). Immunity. 25(3); 349-360).

特異性

Clone AR1 is a mouse monoclonal antibody that detects Interferon regulatory factor 3 (IRF-3). It targets an epitope within the DNA-binding domain from the N-terminal half.

免疫原

His-tagged full-length human recombinant human Interferon regulatory factor 3 (IRF-3).

應用

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-3 in A549 cell lysate.

Tested Applications

ELISA Analysis: A representative lot detected IRF-3 in ELISA applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Flow Cytometry Analysis: A representative lot detected IRF-3 in Flow Cytometry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Western Blotting Analysis: A representative lot detected IRF-3 in Western Blotting applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Immunocytochemistry Analysis: A representative lot detected IRF-3 in Immunocytochemistry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IRF-3, clone AR-1, Cat. No. MABF2778, is a mouse monoclonal antibody that detects Interferon regulatory factor 3 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1