ABE1836

Anti-SMARCAL1 Antibody

serum, from rabbit

• 别名: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, hHARP, Sucrose nonfermenting protein 2-like 1, SMARCAL1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human, mouse
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_001120679
• UniProt登錄號: Q9NZC9
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SMARCAL1(50485)

说明

一般說明

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (UniProt Q9NZC9; also known as ATP-driven annealing helicase, HepA-related protein, hHARP, SMARCA-like protein 1, Sucrose nonfermenting protein 2-like 1) is encoded by the SMARCAL1 (also known as HARP, HHARP) gene (Gene ID 50485) in human. The annealing helicase SMARCAL1 is a replication stress response protein that maintains genome integrity at stalled replication forks during DNA replication. SMARCAL1 binds Replication protein A (RPA) via its RPA-binding motif, which is both necessary and sufficient to target SMARCAL1 to stalled replication forks. Although not necessary for SMARCAL1 enzymatic activity, RPA binding is critical for its cellular function. Ataxia-telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) phosphorylate SMARCAL1 in response to replication stress. SMARCAL1-deficient cells are hypersensitive to replication stress agents and SMARCAL1 gene mutations cause Schimke immunoosseous dysplasia (SIOD).

特異性

Reacts with both human and murine SMARCAL1. Expected to react with all four spliced isoforms of murine SMARCAL1.

免疫原

Recombinant protein corresponding to the N-terminus of human SMARCAL1.

應用

Anti-SMARCAL1 Antibody, Cat. No. ABE1836, is a highly specific rabbit Polyclonal antibody, that targets SMARCAL1 and has been tested in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 3.0 µL from a representative lot immunoprecipitated SMARCAL1 in HEK293T cell lysate (Data courtesy of Dr. David Cortez, Vanderbilt University).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SMARCAL1 in siRNA transfected HEK293T cell lysate (Data courtesy of Dr. David Cortez, Vanderbilt University).
Western Blotting Analysis: A representative lot detected SMARCAL1 in U2OS cell lysate (Bansbach, C.E., et al. (2009). Genes Dev. 23(20):2405-2414).

品質

Evaluated by Western Blotting in mouse myoblast C2C12 nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SMARCAL1 in 10 µg of mouse myoblast C2C12 nuclear extract.

標靶描述

~110 kDa observed. Human SMARCAL1 (105.9 kDa calculated). Mouse SMARCAL1 (100.8 kDa/isoform 1, 87.1 kDa/isoform 2, 63.2 kDa/isoform 3, 44.2 kDa/isoform 4 calculated). Uncharacterized band(s) may appear in some lysates.

其他說明

Concentration: Please refer to lot specific datasheet.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1