AB110-I

Anti-PNMT Antibody

from rabbit, purified by affinity chromatography

• 别名: Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001254480
• UniProt登錄號: Q9UBM1
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PEMT(10400)

说明

一般說明

PNMT, also known as Phosphatidylethanolamine N-methyltransferase, or PEMT, PEAMT, or PEMT2, and encoded by the gene PEMT/PEMPT/PNMT, catalyzes three sequential methylation reactions of phosphatidylethanolamine to produce phosphatidylcholine a major constituent of cell membranes and a primary pulmonary surfactant. PNMT/PEMT is localized to the endoplasmic reticulum membrane as well as the mitochondrial membranes. PNMT/PEMT is widely expressed, and has the highest activity in the mitochondria. The conversion of phosphatidylethanolamine to phosphatidylcholine by sequential methylation via PNMT/PEMT occurs in the liver. A different pathway for phosphatidylcholine production starting from choline also is present in cells but it does not involve PNMT/PEMT enzyme. Mutations in PNMT/PEMT protein are associated with nonalcoholic fatty liver disease (NAFLD).

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human PNMT.

應用

Anti-PNMT Antibody detects level of PNMT & has been published & validated for use in PNMT.

Immunohistochemistry Analysis: A 1:500 dilution and a 1:2,000 dilution from a representative lot detected PNMT in human adrenal gland and testis tissues, respectively.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

品質

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PNMT in 10 µg of human liver tissue lysate.

標靶描述

~17 kDa observed. As isoform of PNMT has been observed between 15-20 kDa (Cui, Z., et al. (1993). J Biol. Chem. 268(22):16655-16663.).

聯結

Replaces: AB110

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable