推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
20G3, monoclonal
物種活性
human, mouse
製造商／商標名
Upstate®
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (non-phospho-Tyr1173)
基因資訊
human ... EGFR(1956)
mouse ... Egfr(13649)
特異性
The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.
免疫原
peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor
應用
Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
品質
routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells
標靶描述
175kDa
聯結
Replaces: 04-337; 04-341
外觀
Thiophilic adsoption and size exclusion chromatography
100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
儲存和穩定性
2 years at -20°C
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
Gang Chen et al.
Biological procedures online, 13, 1-1 (2011-03-04)
Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR
Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
Sorkin, A, et al.
The Journal of Biological Chemistry, 267, 8672-8678 (1992)
Alteration of epidermal growth factor receptor activity by mutation of its primary carboxyl-terminal site of tyrosine self-phosphorylation.
Bertics, P J, et al.
The Journal of Biological Chemistry, 263, 3610-3617 (1988)
Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice.
Siggs, OM; Grieve, A; Xu, H; Bambrough, P; Christova, Y; Freeman, M
Biology Open null
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