05-163
Anti-PLCγ-1 Antibody
Upstate®, from mouse
别名:
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1, Phosphoinositide phospholipase C, Phospholipase C-gamma-1, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phospholipase C gamma 1,
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所有图片(2)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
B-2-5, monoclonal
B-20-3, monoclonal
B-6-4, monoclonal
D-7-3, monoclonal
E-9-4, monoclonal
物種活性
mouse, human, bovine, rat, rabbit
製造商／商標名
Upstate®
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PLCG1(5335)
一般說明
Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
特異性
This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).
免疫原
Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.
應用
Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.
Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.
Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.
Research Category
Signaling
Signaling
Research Sub Category
Lipid Signaling
Lipid Signaling
品質
Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.
標靶描述
135 kDa
外觀
Protein G Chromatography
Format: Purified
Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as purified immunoglobulin. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as purified immunoglobulin. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.
儲存和穩定性
Stable for 1 year at -20ºC from date of shipment.
分析報告
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
A Kosugi et al.
Immunity, 14(6), 669-680 (2001-06-23)
To elucidate the process of TCR-mediated signaling pathways in lipid rafts, we constructed a chimeric molecule that localizes activated SHP-1 to rafts. Raft targeting of activated SHP-1 in Jurkat-derived transfectants completely inhibited the expression of CD69 and transcriptional factors after
Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida.
S J MacKenzie,N A Peres,M P Barquero,L F Arauz,L W Timmer
Phytopathology null
L M Mehlmann et al.
Developmental biology, 203(1), 221-232 (1998-11-10)
The initiation of Ca2+ release at fertilization of mammalian eggs requires inositol trisphosphate (Miyazaki et al., 1992, Science 257, 251-255), indicating that an enzyme of the phospholipase C family is probably activated. Because Ca2+ release at fertilization in echinoderm eggs
Thrombin stimulates phosphorylation of insulin-like growth factor-1 receptor, insulin receptor substrate-1, and phospholipase C-gamma 1 in rat aortic smooth muscle cells.
Rao, G N, et al.
The Journal of Biological Chemistry, 270, 27871-27875 (1995)
J M Haugh et al.
The Journal of biological chemistry, 274(13), 8958-8965 (1999-03-20)
The epidermal growth factor receptor (EGFR) ligands, epidermal growth factor (EGF), and transforming growth factor-alpha (TGFalpha) elicit differential postendocytic processing of ligand and receptor molecules, which impacts long-term cell signaling outcomes. These differences arise from the higher affinity of the
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