推荐产品
形狀
chloroform solution
包裝
pkg of 2 × 20 mL (100600C-1g)
pkg of 1 × 4 mL (100600C-100mg)
pkg of 5 × 4 mL (100600C-500mg)
製造商／商標名
Avanti Research™ - A Croda Brand
濃度
25 mg/mL (100600C-100mg)
25 mg/mL (100600C-1g)
25 mg/mL (100600C-500mg)
脂質類型
lipid extracts
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.
應用
E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.
E. coli extract polar has been for the preparation of liposomes.
E. coli Extract Polar has been used:
- in unilamellar liposomes preparation for kinetic studies
- in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies
- in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies
生化／生理作用
E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. It is used for reconstituting membrane proteins and forms bilayer by vesicle fusion.
包裝
30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)
5 mL Clear Glass Sealed Ampule (100600C-100mg)
5 mL Clear Glass Sealed Ampule (100600C-500mg)
法律資訊
Avanti Research is a trademark of Avanti Polar Lipids, LLC
也與該產品經常一起購買
产品编号
说明
价格
訊號詞
Danger
危險分類
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
標靶器官
Central nervous system, Liver,Kidney
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
水污染物質分類（WGK）
WGK 3
閃點（°F）
does not flash
閃點（°C）
does not flash
其他客户在看
Methods in Cell Biology, 128, 223-241 (2015)
Small substrate transport and mechanism of a molybdate ATP binding cassette transporter in a lipid environment
Rice AJ, et al.
The Journal of biological chemistry, 289(21), 15005-15013 (2014)
An inducible reconstitution system for the real-time kinetic analysis of protease activity and inhibition inside the membrane
Baker RP and Urban S
Methods in Enzymology, 584, 229-253 (2017)
The
Enzymology Primer for Recombinant DNA Technology (2017)
Effects of lipid environment on the conformational changes of an ABC importer
Rice AJ, et al.
Channels (Austin, Tex.), 8(4), 327-333 (2014)
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