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Stable Isotopes

Image showing scientist using stable isotope-labeled biomolecules in lab

ISOTEC® Stable Isotopes are stable isotope compounds, ranging from gases to complex molecules. These compounds are useful for tracer studies in proteomics and metabolomics, agents for MRI / MRS, and in a wide range of other biomedical applications. 

Both tracer and flux studies benefit from stable isotope label assistance. Metabolic pathways can be studied by tracking naturally-occurring and disease-indicated metabolites. Additionally, the effects of exogeneous substances or perturbations on the metabolome can be elucidated with the use of our stable isotope-labeled internal standards coupled with mass spectroscopy determination.  

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Applications for Stable Isotopes

The use of our stable isotope-labeled compounds in metabolic and nutritional studies provides a means to dissect biochemical pathways. The availability of isotopically labeled compounds for basic scientific research permits the examination of the role of metabolic pathways in maintaining physiological states. In vivo studies using stable isotope labeled metabolites reveal important aspects of metabolism, including pathways and flux.

An important tool for high-resolution structure determination by NMR spectroscopy is the use of stable isotope-labeled biomolecules. By either selectively or uniformly incorporating stable isotopes into proteins, investigators can reduce the complexity of their spectra significantly. 13C, 15N, and deuterium are the most common isotopes incorporated into proteins through microbial expression systems, peptide synthesis, or with cell-free extracts.

The use of stable isotope compounds in medical imaging, in conjunction with 13C-MRS in vivo techniques, provides a method to explore fluxes through energy-related metabolic pathways for early diagnosis and treatment of neuropsychiatric disorders, cancer, and other diseases.

For these and many other applications, we offer a comprehensive portfolio of isotope labeled carbohydrates, amino acids, protected amino acids, metabolites, fatty acids, vitamins, steroids, other biologically-active compounds, and cell culture media and components. We also provide isotopically-labeled synthetic building blocks for researchers who prefer to make their own compounds. With our expertise in isotope synthesis, we stand ready to provide custom compounds for all of your specific needs.

Related Applications
Peptide Synthesis
Peptide synthesis involves the formation of a peptide bond between two amino acids to create a peptide composed of a chain of multiple amino acids.
Wet Chemical Analysis
The wet chemical analysis involves identifying and quantifying the desired elements present in a liquid sample using several measuring techniques. With chemical reagents, the analyte converts a dye proportionally, which can be read-out visually or photometrically.
Polymer Synthesis
Polymer synthesis, or polymerization, is the process of joining small molecule monomers through chemical reactions to produce large polymer molecules that consist of long chains or networks of monomers linked together by covalent bonds.
Protein Labeling & Modification
Protein labeling applications using enzyme-protein labeling, fluorescent protein labeling, and targeted protein degradation technologies.
Cancer Research
Cancer research strives to not only identify the cause, but also methods for detection, treatment, and prevention. Learn more about our contributions to cancer research in academic and industrial settings.
Metabolomics Research
Metabolomics research is the study of the metabolome, or complete set of metabolites, in biological systems by identifying metabolomic pathways for pharmaceutical and diagnostic, genomics, nutritional, and environmental applications.
Mass Spectrometry
Mass spectrometry (MS) is an analytical tool used to identify compounds, determine chemical structure, and assess isotopic abundance. In MS, samples are ionized, and the resulting ions are identified based on their mass-to-charge (m/z) ratios.
Nuclear Magnetic Resonance (NMR)
NMR spectroscopy is an analytic technique to determine molecular structure, chemical composition and purity. NMR detects the energy absorbed due to nuclear spin states in the presence of a strong magnetic field.
Related Categories
Reference Materials
Chemical reference standards for chromatography (HPLC, GC), spectroscopy (AAS, ICP), physical properties for applications in pharmaceutical, environmental, petrochemical, food and beverage, forensics, and trace analysis.
Vitamin Standards
Discover our selection of all water- and fat-soluble vitamin standards, from analytical standard to pharmaceutical reference standard purity grade for your various applications involving HPLC and GC instruments.
Biomarker & Metabolite Standards
Explore our wide selection of reference standards of hormones, hydroxyvitamin D, amino acid metabolites, and catecholamines for biomarker analysis and metabolomics.
Bioprocessing Cell Culture Media
Our off-the-shelf and customizable bioprocessing cell culture media (CCM) products enhance productivity in upstream mAb, vaccine, gene/cell therapy processes.
Avanti® Polar Lipids
We provide Avanti® Polar Lipids high-purity lipids, adjuvants, and equipment for lipidomics research solutions for complex therapeutic drugs and next generation mRNA vaccines.
Metabolism Assay Kits
We offer a broad range of metabolism assay kits for the convenient and dependable analysis of metabolites and enzymes for all your research applications.
Metabolite Libraries
We provide small molecule metabolite libraries and kits with a comprehensive range of primary metabolite compounds covering key metabolic pathways for your metabolomics research.
Oligos, qPCR Probes & Peptides
Choose custom products from our comprehensive portfolio of DNA and RNA oligos, qPCR probes and peptides as we offer numerous options for manufacturing platforms, purity levels, modifications to meet a variety of research applications.

ISOTEC® Stable Isotopes Quotation Request

Many of our stable isotope products are available from bulk stock. To receive pricing, availability and packaging information, complete the form below. You may also email your request to isosales@milliporesigma.com or contact the Stable Isotope Customer Service Team.  

Fields indicated by an * are required. 

Related Product Resources

Biomolecular NMR

Isotope Labeling Methods for Protein Dynamics Studies
Isotopic Labeling for NMR Spectroscopy of Biological Solids
Methyl Isotope-Labeling of Proteins
Utilizing ISOGRO® Supplementation of M9 Minimal Media
Selective Protonation of Methyl Groups in Highly Deuterated Proteins
Solid-state NMR on Larger Biomolecules
Application Note: a-Ketoacids as Biosynthetic Precursors for Protein NMR
Application Note: Isotopic Labeling in Solid-state NMR of Proteins
Application Note: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
Technical Bulletin: Utilizing ISOGRO® as Stand-alone or Supplemental Media

Mass Spec

Isotopically Labeled Steroid Standards
Application Note: Comparison of Deuterium, 13C, and 15N Isotopic Labels in Mass Spec Standards

MRI / MRS / Hyperpolarization / PET

Parahydrogen Induced Polarization
13C MRE to Probe Brain Metabolism Using Hyperpolarized Pyruvic Acid
Dynamic Nuclear Polarization
MRI/MRS: Use of Isotopes in Hyperpolarization
Application Note: The Essential Role of Enriched Stable Nuclides in Positron Emission Tomography (PET)

Metabolism Research

Stable Isotope Labeled Fatty Acids as Metabolic Tracers
Tools for Measuring Energy Expenditure
Application Note: An Approach to Quantifying the conversion of Carbohydrate to Fat in Humans
Brochure: Metabolomics

Related Services
Stable Isotope Custom & GMP Products
Our Isotec® Stable Isotopes team offers custom synthesis and cGMP services to design and manufactures complex labeled chemicals for a variety of research applications.

Highlights

Metabolic Imaging with Hyperpolarization
Metabolic Imaging with Hyperpolarization

13C labeled metabolites detection can render MRI images of metabolic changes in the body with the goal of diagnosis of cancer and other diseases.

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