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TRUECD

Sigma-Aldrich

TrueGel3D Crosslinker

CD cell-degradable crosslinker

Synonym(s):

Crosslinker

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.52

shipped in

dry ice

storage temp.

−20°C

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General description

CD cell-degradable crosslinker is comprised of conjugated peptide along with polyethylene glycol and thiol (SH) groups. The thiol groups form stable thioether bond with thiol-reactive groups of the polymers (Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran) and results in a stable hydrogel.

The conjugated peptide has matrix metalloprotease (MMP)-cleavage sites (Pro-Leu-Gly-Leu-Trp-Ala), which allows matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9) to locally degrade the polymer network. It allows cells to spread and migrate within the gel if they express the indicated MMPs.

Application

TRUECD is used to crosslink PVA and dextran polymers to generate cell-degradable hydrogel

Features and Benefits

MMP degradable

Components

TRUECD contains CD cell-degradable crosslinker lyophilized and water.

Caution

Avoid prolong exposure of CD cell-degradable crosslinker to air

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

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Articles

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

Protocols

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.

TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits

Related Content

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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