R306-05A

Rat Cardiac Fibroblasts: RCF, adult

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: rat heart (Sprague Dawley, normal adult)
• Quality Level: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 42
• morphology: fibroblast
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. They are known to play key roles in the development of cardiac disease and hypertrophy. Rat Cardiac Fibroblasts provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.

Cell Line Origin

Heart

Application

human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines

Components

Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 8 population doublings

Subculture Routine

Please refer to the RCF Culture Protocol.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable