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GE17-0981-01

CNBr Activated Sepharose™ 4 Fast Flow

Cytiva 17-0981-01, pack of 10 g

Synonym(s):

Affinity chromatography resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

cyanogen bromide

packaging

pack of 10 g

manufacturer/tradename

Cytiva 17-0981-01

storage condition

(Unsused: 2 - 8°C - Used: 2 - 8°C in 20% Ethanol)

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-11(Ligand dependent)

working range

3-11(ligand dependent)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

General description

CNBr-activated Sepharose™ 4 Fast Flow is a well established, pre-activated chromatography medium for coupling of large amino-containing ligands.

CNBr-activated Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Speharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups.

The preparation and use of affinity chromatography media by coupling biospecific ligands to CNBr-activated Sepharose™ 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.

CNBr-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, CNBr-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands.
  • Easy, rapid and efficient coupling
  • This resin allows for multi-point attachment of protein ligands which minimizes ligand leakage
  • BioProcess medium supported for industrial applications and well-established in approved processes

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Coupling through the Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service