C6805
Monoclonal Anti-Collagen, Type VII antibody produced in mouse
clone LH7.2, ascites fluid
Synonym(s):
Anti-EBD1, Anti-EBDCT, Anti-EBR1, Anti-NDNC8
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LH7.2, monoclonal
contains
15 mM sodium azide
species reactivity
human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine
technique(s)
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COL7A1(1294)
Related Categories
General description
Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type VII is the crucial component of anchoring fibrils and mutation in type VII collagen gene (COL7A1) leads to epidermolysis bullosa dystrophica. Monoclonal anti-collagen, type VII antibody can be used to distinguish invasive melanoma from non-invasive melanoma by visualizing the appearance and integrity of epidermal basement membrane. It can also be used in immunoblotting. Mouse anti-collagen type VII antibody reacts specifically with an epitope located on collagenase digested type VII collagen (150 kDa). The product also reacts with the basement membrane zone of stratified squamous epithelia of sheep, pig, bovine, guinea pig, goat and human.
Collagen type VII alpha 1 chain (COL7A1) is majorly expressed in skin, secreted by keratinocytes and fibroblasts. The gene is located on human chromosome 3p21.31.
Immunogen
insoluble fractions of neonatal human foreskin epidermal cells
Application
Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in immuno-gold labelling.
Monoclonal anti-collagen type VII antibody can be used as a primary antibody in virus titration. It can also be used for the localization of type VII collagen in different immunochemical assays like dot blot, immunocytochemistry and ELISA.
Biochem/physiol Actions
Collagen type VII alpha 1 chain (COL7A1) is required for epithelium-to-stroma anchorage in skin, mucosa and cornea. It is considered as an immediate-early response gene for transforming growth factor β (TGF-β)/SMAD (mothers against decapentaplegic) signaling pathway.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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R Schucht et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 14(2), 285-292 (2006-05-16)
We developed a new strategy that provides well-defined high-titer producer cells for recombinant retroviruses in a minimum amount of time. The strategy involves the targeted integration of the retroviral vector into a chromosomal locus with favorable properties. For proof of
Molecular basis of dystrophic epidermolysis bullosa: mutations in the type VII collagen gene (COL7A1).
Jarvikallio A, Pulkkinen L
Human Mutation, 10(5), 338-347 (1997)
Type VII Collagen in the Human Accommodation System: Expression in Ciliary Body, Zonules, and Lens Capsule
Wullink B, et al.
Investigative Ophthalmology & Visual Science, 59(2), 1075-1083 (2018)
SMAD3/4-dependent transcriptional activation of the human type VII collagen gene (COL7A1) promoter by transforming growth factor ?
Vindevoghel L, et al.
Proceedings of the National Academy of Sciences of the USA, 95(25), 14769-14774 (1998)
R H Wetzels et al.
The American journal of pathology, 139(2), 451-459 (1991-08-01)
The distribution of basement membrane type VII collagen was detected immunohistochemically and compared in normal human organs and their neoplastic derivatives using monoclonal antibody LH7.2. In normal tissues, type VII collagen was found to be restricted to the basement membrane
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