494460
Octyl β-D-glucopyranoside
ULTROL® Grade, ≥99% (TLC), non-ionic detergent to solubilize membrane-bound proteins in their native state
Synonym(s):
n-Octyl-β-D-glucopyranoside, ULTROL® Grade, OG, n-Octylglucoside
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About This Item
Empirical Formula (Hill Notation):
C14H28O6
CAS Number:
Molecular Weight:
292.37
MDL number:
UNSPSC Code:
12161902
NACRES:
NA.21
Recommended Products
Quality Level
Assay
≥99% (TLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
aggregation number
84
impurities
≤0.05% n-octanol
color
white
CMC
20 - 25 mM
solubility
water: 100 mg/mL
shipped in
ambient
storage temp.
15-25°C
InChI
1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
InChI key
HEGSGKPQLMEBJL-RKQHYHRCSA-N
General description
A highly purified nonionic detergent intended for solubilizing membrane-bound proteins in their native state, and for the preparation of lipid vesicles. Its well-defined chemical structure, small uniform micelles, and high water solubility make it superior to most other nonionic detergents for membrane solubilization. Because of its high critical micelle concentration (20-15 mM), it has the additional advantage of being removed easily by dialysis when compared to bile salts. Aggregation number: 84.
Absorbance (10%, H2O, 260 nm):≤0.3; conductivity (100 mM, H2O):≤100 µmhos.
Highly purified non-ionic detergent intended for solubilizing membrane-bound proteins in their native state. Aggregation number: 84.
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, store in the refrigerator (4°C) under sterile conditions. Aqueous stock solutions are stable for 3 months at 4°C. Solutions are susceptible to hydrolysis (especially in strong acid) and enzymatic degradation.
Other Notes
Hoshino, T., et al. 1992. J. Biol. Chem.267, 21313.
Levy, D., et al. 1992. Biochim. Biophys. Acta1107, 283.
Nunn, R.S., et al. 1992. J. Mol. Biol.228, 1259.
Lorber, B., et al. 1990. Biochim. Biophys. Acta1023, 254.
Jopski, B., et al. 1989. Biochim. Biophys. Acta978, 79.
Camm, E.L., et al. 1981. Plant Physiol.67, 1061.
Gould, R.J., et al. 1981. Biochemistry20, 6776.
Levy, D., et al. 1992. Biochim. Biophys. Acta1107, 283.
Nunn, R.S., et al. 1992. J. Mol. Biol.228, 1259.
Lorber, B., et al. 1990. Biochim. Biophys. Acta1023, 254.
Jopski, B., et al. 1989. Biochim. Biophys. Acta978, 79.
Camm, E.L., et al. 1981. Plant Physiol.67, 1061.
Gould, R.J., et al. 1981. Biochemistry20, 6776.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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B Lorber et al.
Biochimica et biophysica acta, 1023(2), 254-265 (1990-04-13)
The commercial non-ionic detergent octyl beta-D-glucopyranoside is often contaminated by significant amounts of UV absorbing and/or ionic compounds that can associate with membrane proteins. Such impurities can be monitored by several techniques (i.e., spectrophotometry, size exclusion chromatography, and pH, conductivity
B Jopski et al.
Biochimica et biophysica acta, 978(1), 79-84 (1989-01-16)
Hemoglobin (Hb) was encapsulated in phosphatidylcholine vesicles by removal of the detergent n-octyl beta-D-glucoside (OG) or n-octyltetraoxyethylene (C8E4) out of mixed detergent-lipid micelles in Hb solution. Three types of apparatus were used for dialysis. Dialysis buffer flow rates, the surface
E L Camm et al.
Plant physiology, 67(5), 1061-1063 (1981-05-01)
The use of the non-ionic detergent octyl beta-d-glucoside has allowed the demonstration in spinach of a chlorophyll a+b-protein complex of apparent molecular weight 29 kilodaltons (Camm and Green, 1980, Plant Physiol 66: 428-432). Complexes analogous to this one also can
T Hoshino et al.
The Journal of biological chemistry, 267(30), 21313-21318 (1992-10-25)
The high affinity branched-chain amino acid transport system (LIV-I) in Pseudomonas aeruginosa is composed of five components: BraC, a periplasmic binding protein for branched-chain amino acids; BraD and BraE, integral membrane proteins; BraF and BraG, putative nucleotide-binding proteins. By using
R S Nunn et al.
Journal of molecular biology, 228(4), 1259-1262 (1992-12-20)
The LH1 light harvesting complex has been purified from a mutant of the photosynthetic bacterium Rhodobacter sphaeroides which synthesizes LH1 as the sole pigment protein. Crystallization trials using polyethylene glycol as the precipitant in the presence of the detergent n-octyl
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