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SRE0095

Sigma-Aldrich

Fast β-Glucuronidase, Recombinant

from limpets (Patella Vulgata), aqueous solution

Synonym(s):

β-Glucuronidase from limpets (Patella vulgata), β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
Enzyme Commission number:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in proprietary host

Quality Level

200

form

aqueous solution

concentration

300000-400000 units/mL

shipped in

ambient

storage temp.

2-8°C

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Application

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Features and Benefits

Formulated with a higher enzyme concentration for applications requiring ultra-fast hydrolysis rates. Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Physical form

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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