HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell Glycan using HILIC-FLR
Materials
analytical column
CONDITIONS
column
HPLC BIOshell Glycan Column, 15 cm x 2.1 mm I.D., 2.7 μm particles (50994-U)
mobile phase
A: 50 mM ammonium formate (CAt. No. 17843) pH 4.4, [ammonium] = 50 mM, pH adjust with formic acid (Fluka 94318) B: Acetonitrile (Sigma-Aldrich 34851)
gradient
75% to 59% B in 75 min
flow rate
0.3 mL/min
detector
FLR (308 nm excitation, 359 emission)
injection
10 μL
sample
Dextran Ladder (31417) after Procainamide Labeling Protocol
Description
Analysis Note
The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. When analyzed on the BIOshell Glycan HPLC Column, this standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide. This ladder provides calibration reference points that can aide in identifying more complex glycans based upon retention characteristics.
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