HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V
Materials
analytical column
CONDITIONS
column
CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase
[A] ethanol; [B] hexane; (25:75, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 205 nm
injection
10 μL
sample
1 mg/mL in methanol
Description
Analysis Note
Methylphenobarbital (also known as mephobarbital and mephobarbitone) is a barbiturate derivative used primarily as an anticonvulsant, but also as a sedative and anxiolytic. It is a chiral compound and the enantiomers have been shown to exhibit different bioactivity, one narcotic, one convulsant, neither of which is seen in the racemate. Shown here is the rapid, efficient separation of methylphenobarbital enantiomers on an Astec CHIROBIOTIC V column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. A Sigma-Aldrich USP reference standard provided reliable identification and quantification.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC