GC Analysis of FAMEs in Cookies on SP®-2560 after SPE Fractionation using Discovery® Ag-Ion
Materials
analytical column
SPE tube or plate
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09
SPE tube/cartridge
Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225-U)
condition
4 mL of acetone; allow solvent to gravity drip completely through tube; discard eluant; 4 mL of hexane; allow solvent to gravity drip completely through tube; discard eluant
sample addition
1 mL of extract; discard any eluant that drips through tube
elution
(Fraction 1) 6 mL of hexane:acetone (96:4); collect eluant in a fresh container with slight vacuum; ((Fraction 2) 4 mL of hexane: acetone (90:10); collect eluant in a fresh container with slight vacuum;(Fraction 3) 4 mL of 100% acetone; collect eluant in a fresh container with slight vacuum)
eluate post-treatment
evaporate each fraction at room temperature using nitrogen; reconstitute each fraction to 1 mL of hexane
column
SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)
oven
180 °C
inj. temp.
250 °C
detector
FID, 250 °C
carrier gas
hydrogen, 1 mL/min
injection
1 μL, 10:1 split
liner
4 mm I.D., split type, single taper wool packed FocusLiner™ design
Description
Analysis Note
A sample of commercially purchased cookies were prepared using an acid digestions /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (1). The resulting extracts were then fractionated using Ag-ion SPE and analyzed by GC/FID on the 100 m SP-2560 capillary column.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SP is a registered trademark of Sigma-Aldrich Co. LLC