Skip to Content
Merck
  • Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations.

Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations.

Parasites & vectors (2015-05-30)
Marcelo Ramalho-Ortigão, Iliano V Coutinho-Abreu, Valdir Q Balbino, Carlos Alberto S Figueiredo, Rami Mukbel, Hussan Dayem, Hanafi A Hanafi, Shabaan S El-Hossary, Emad El-Din Y Fawaz, Mahmoud Abo-Shehada, David F Hoel, Gwen Stayback, Mariha Wadsworth, Douglas A Shoue, Jenica Abrudan, Neil F Lobo, Andrew R Mahon, Scott J Emrich, Shaden Kamhawi, Frank H Collins, Mary Ann McDowell
PMID26022221
ABSTRACT

The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines. Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein. The analysis of PpSP15 expression from field populations revealed significant intra- and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections. Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.

MATERIALS
Product Number
Brand
Product Description
S5505
Sigma-Aldrich
Silica, fumed, powder, 0.2-0.3 μm avg. part. size (aggregate)
85356
Sigma-Aldrich
Silicon dioxide, granular, ≥99.9%
N7000
Sigma-Aldrich
1-Naphthyl phosphate monosodium salt monohydrate, ≥98% (titration), powder
560979
Sigma-Aldrich
Silica, mesostructured, MSU-F (cellular foam)
S5130
Sigma-Aldrich
Silica, fumed, powder
421553
Sigma-Aldrich
Silica
381276
Supelco
Silica, 99.8%
420808
Sigma-Aldrich
LUDOX® LS colloidal silica, 30 wt. % suspension in H2O
420786
Sigma-Aldrich
LUDOX® TM-40 colloidal silica, 40 wt. % suspension in H2O
420794
Sigma-Aldrich
LUDOX® SM colloidal silica, 30 wt. % suspension in H2O
342890
Sigma-Aldrich
Silicon dioxide, −325 mesh, 99.5% trace metals basis
342831
Sigma-Aldrich
Silicon dioxide, fused (granular), 4-20 mesh, 99.9% trace metals basis
777242
Sigma-Aldrich
Silica, mesostructured, SBA-15, 99% trace metals basis
643645
Sigma-Aldrich
Silica, mesostructured, MCM-41 type (hexagonal)
634867
Sigma-Aldrich
Silicon dioxide, single crystal substrate, optical grade, 99.99% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm
637238
Sigma-Aldrich
Silicon dioxide, nanopowder, 10-20 nm particle size (BET), 99.5% trace metals basis
637246
Sigma-Aldrich
Silicon dioxide, nanopowder (spherical, porous), 5-20 nm particle size (TEM), 99.5% trace metals basis
748161
Sigma-Aldrich
Silica, nanoparticles, mesoporous, 200 nm particle size, pore size 4 nm
440345
Supelco
Glass spheres
718483
Sigma-Aldrich
Silica, nanopowder, 99.8% trace metals basis
420824
Sigma-Aldrich
LUDOX® HS-30 colloidal silica, 30 wt. % suspension in H2O
420883
Sigma-Aldrich
LUDOX® CL colloidal silica, 30 wt. % suspension in H2O
420840
Sigma-Aldrich
LUDOX® AS-40 colloidal silica, 40 wt. % suspension in H2O
420832
Sigma-Aldrich
LUDOX® AS-30 colloidal silica, 30 wt. % suspension in H2O
420859
Sigma-Aldrich
LUDOX® TMA colloidal silica, 34 wt. % suspension in H2O
420875
Sigma-Aldrich
LUDOX® AM colloidal silica, 30 wt. % suspension in H2O
420891
Sigma-Aldrich
LUDOX® CL-X colloidal silica, 45 wt. % suspension in H2O
420778
Sigma-Aldrich
LUDOX® TM-50 colloidal silica, 50 wt. % suspension in H2O
420816
Sigma-Aldrich
LUDOX® HS-40 colloidal silica, 40 wt. % suspension in H2O
204358
Sigma-Aldrich
Sand, white quartz, ≥99.995% trace metals basis