Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Toxicology mechanisms and methods (2015-01-07)

E Brindha, M Rajasekapandiyan

ABSTRACT

This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

MATERIALS

Product Number

Brand

Product Description

M8266

Sigma-Aldrich

Magnesium chloride, anhydrous, ≥98%

M4880

Sigma-Aldrich

Magnesium chloride, BioReagent, suitable for insect cell culture, ≥97.0%

P9625

Sigma-Aldrich

Phenazine methosulfate

B4875

Sigma-Aldrich

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%

05030

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H₂O

241393

Sigma-Aldrich

N-Phenyl-p-phenylenediamine, 98%

208337

Sigma-Aldrich

Magnesium chloride, powder, <200 μm

449164

Sigma-Aldrich

Magnesium chloride, AnhydroBeads^™, −10 mesh, 99.9% trace metals basis

449172

Sigma-Aldrich

Magnesium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H₂O

Y0000620

Sodium laurilsulfate, European Pharmacopoeia (EP) Reference Standard

S5886

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

H1758

Sigma-Aldrich

Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

S6150

Sigma-Aldrich

Sodium chloride, tablet

S3014

Sigma-Aldrich

Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

2104

Supelco

Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

M8787

Sigma-Aldrich

Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂solution for PCR

M1028

Sigma-Aldrich

Magnesium chloride solution, for molecular biology, 1.00 M±0.01 M

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

H9892

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

S1679

Sigma-Aldrich

Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

S7653

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

17934

Supelco

Hydrogen chloride – ethanol solution, ~1.25 M HCl, for GC derivatization, LiChropur^™