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Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Artificial cells, nanomedicine, and biotechnology (2013-06-26)

Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç

ABSTRACT

In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

D-Glucose-¹²C₆, ¹⁶O₆, 99.9 atom % ¹⁶O, 99.9 atom % ¹²C

Supelco

D-(+)-Glucose, analytical standard

Sigma-Aldrich

Urea solution, BioUltra, ~8 M in H₂O

Sigma-Aldrich

L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)

Supelco

L-Ascorbic acid, analytical standard

Sigma-Aldrich

Urea-¹²C, 99.9 atom % ¹²C

Sigma-Aldrich

L-Ascorbic acid, ACS reagent, ≥99%

Sigma-Aldrich

D-(+)-Glucose, Hybri-Max^™, powder, BioReagent, suitable for hybridoma

Sigma-Aldrich

D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)

Sigma-Aldrich

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

Sigma-Aldrich

D-(+)-Glucose, tested according to Ph. Eur.

Sigma-Aldrich

Urea solution, 40 % (w/v) in H₂O

Supelco

Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

Ascorbic Acid, Pharmaceutical Secondary Standard; Certified Reference Material

USP

Ascorbic acid, United States Pharmacopeia (USP) Reference Standard

Millipore

Urea solution, suitable for microbiology, 40% in H₂O

Sigma-Aldrich

Sodium phosphate monobasic dihydrate, purum p.a., crystallized, ≥99.0% (T)

Sigma-Aldrich

L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)

Sigma-Aldrich

L-Ascorbic acid, BioUltra, ≥99.5% (RT)

Sigma-Aldrich

D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)

Sigma-Aldrich

L-Ascorbic acid, tested according to Ph. Eur.

USP

Dextrose, United States Pharmacopeia (USP) Reference Standard

Sigma-Aldrich

Sodium phosphate monobasic dihydrate, BioUltra, for molecular biology, ≥99.0% (T)

Supelco

L-Ascorbic acid, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Sigma-Aldrich

Sodium phosphate monobasic dihydrate, meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, 98-100.5% (calc. to the dried substance)