- Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis.
Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis.
Bio-protocol (2021-03-05)
Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J Meikle, Dmitri Sviridov
PMID33659340
ABSTRACT
Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.
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