ZRB2626

Anti-Trypsin Antibody, clone 1F6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Anionic trypsin I, Beta-trypsin, Cationic trypsinogen, EC 3.4.21.4, Serine protease 1, Trypsin I
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F6, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 26.56 kDa; observed mol wt ~27 kDa
• purified by: using Protein A
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_002760.1
• UniProt accession no.: P07477
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... PRSS1(5644)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Trypsin. It targets an epitope within 15 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Trypsin.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse pancreas tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Trypsin in Mouse pancreas tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Trypsin in Human pancreas and Rat pancreas tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Trypsin in HT-29 cells.

Affinity Binding Assay: A representative lot of this antibody bound Trypsin peptide with a KD of 1.2 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Trypsin in Human pancreas tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Serine protease 1 (UniProt: P07477; also known as EC 3.4.21.4, Anionic trypsin I, Beta-trypsin, Cationic trypsinogen, Trypsin I, Trypsin-1) is encoded by the PRSS1 (also known as TRP1, TRY1, TRYP1) gene (Gene ID: 5644) in human. Trypsin-1 is a secreted serine protease that is expressed in pancreas and several other tissue. It is synthesized with a signal peptide (aa 1-15) and a propeptide (aa 16-23) that are subsequently cleaved off to produce the mature active protein (aa 24-247). It cleaves carboxyl groups present on lysine and arginine residues. Three isoforms of trypsin (trypsin 1, 2, and 3) have been described that are synthesized and stored in the pancreas as inactive trypsinogens. Upon stimulation by cholecystokinin, the pancreas releases inactive trypsinogens into the intestine where they are activated by enterokinase via proteolytic cleavage. It can be present in a single-chain form and a two-chain form that are produced by proteolytic cleavage after arginine 122. Its sulfation at tyrosine 154 is reported to increase its selectivity towards basic versus apolar residues at the P2′ position of inhibitors that bind in a substrate-like fashion. Mutations in PRSS1 gene have been linked to hereditary pancreatitis that is characterized by pancreatic inflammation, permanent destruction of the pancreatic parenchyma, maldigestion, and severe abdominal pain attacks. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pfützer, R., et al. (2002). Gut. 50(2); 271-272; Koshikawa, N., et al. (1994). Biochem. J. 303(Pt1); 187-190).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.