ZRB1503
Anti-Cytochrome c Antibody, clone 1M14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Cyt c, Cytochrome c somatic
Sign Into View Organizational & Contract Pricing
All Photos(24)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1M14, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 11.75 kDa
observed mol wt ~12 kDa
species reactivity
rat, human, monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CYCS(54205)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 1M14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cytochrome c.
Immunogen
GST/His-tagged full-length human recombinant Cytochrome c.
Application
Anti-Cytochrome c, clone 1M14 ZooMAb, Cat. No. ZRB1503, is a recombinant Rabbit monoclonal antibody that detects Cytochrome c and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cytochrome c in HeLa and COS-7 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cytochrome c in HeLa, A431, HUVEC, and NIH 3T3 cells.
Affinity Binding Assay: 100 μg/mL from a representative lot detected Cytochrome c in CYCS protein showed a KD of 2.6 x 10^-8 whereas binding of ZRB1503 to non-specific GST protein showed a KD of 4.4 x 10^-4.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cytochrome c in human heart and human stomach tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cytochrome c in HeLa, A431, HUVEC, and NIH 3T3 cells.
Affinity Binding Assay: 100 μg/mL from a representative lot detected Cytochrome c in CYCS protein showed a KD of 2.6 x 10^-8 whereas binding of ZRB1503 to non-specific GST protein showed a KD of 4.4 x 10^-4.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cytochrome c in human heart and human stomach tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Cytochrome c (UniProt: P99999; also known as Cyt c) is encoded by the CYCS (also known as CYC) gene (Gene ID: 54205) in human. Cytochrome c is a loosely connected inner mitochondrial membrane protein that functions as an electron carrier in the cell respiratory chain by interacting with redox partners of complex III and complex IV. Cytochrome C is highly conserved during evolution. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase and then transfer this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Cytochrome c also plays an important role in apoptosis. It translocates from mitochondria to the cytosol in the cells undergoing apoptosis and participates in the central control or executioner phase of cell death cascade. In this process, caspase-9 and Apaf1 bind to each other in the presence of Cytochrome c and ATP leading to activation of caspase-9, which in turn activates caspase-3 and subsequently DNA fragmentation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service