SRP0138

NSD2 (782-end)/ReBPII Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(s): MMSET, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, REIIBP, Wolf-Hirschhorn syndrome candidate 1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 66.5 kDa
• packaging: pkg of 20 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_133330
• UniProt accession no.: O96028
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... WHSC1(7468)

Description

General description

Human NSD2 (GenBank Accession No. NM_133330), amino acids 782-end with N-terminal DDDDK (FLAG)-His tag, MW = 66.5 kDa, expressed in Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, 100 μg/ml FLAG peptide and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable