PLC/PRF/5
85061113, human liver, epithelial
Synonym(s):
Alexander cell line
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About This Item
UNSPSC Code:
41106514
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product name
PLC/PRF/5, from human liver, 85061113
biological source
human liver
growth mode
Adherent
karyotype
Not specified
morphology
epithelial
products
Hepatitis B Surface Antigen (HBSAg)
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16
Culture Medium
DMEM + 2mM Glutamine + 10% fetal bovine serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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