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Z374873

PCR microtubes with attached caps

capacity 0.2 mL

Synonym(s):

PCR Tubes

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About This Item

UNSPSC Code:
41121702
NACRES:
NB.22

material

colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube

description

Autoclavable

sterility

non-sterile

feature

RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit

packaging

pack of 250 ea
case of 4 packs

technique(s)

PCR: suitable

capacity

0.2 mL

color

clear

suitability

suitable for (PCR applications)

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General description

Thin-walled for efficient thermal transfer and shorter cycle times; fits all leading thermal cyclers including PerkinElmer, Biometra, MJ Research, Techne, Grant and Strategene (0.65 mL only). Certified DNase- and RNase-free. All tolerate organic solvent reactions and temperatures from -4 to 121 °C.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Although quantitative PCR uses the same basic concept as traditional PCR, the reactions differ in that the amplicons are generally smaller and are detected indirectly using an additional dye or labeled probe or primer.

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