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1.01350

Sigma-Aldrich

Leishman′s eosine methylene blue

for microscopy

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About This Item

UNSPSC Code:
41116130
NACRES:
NA.21

Quality Level

400

form

solid

IVD

for in vitro diagnostic use

pH

5-6 (0.01% in methanol: water (1:1))

bulk density

700 kg/m3

εmax

650-850 at 522-525 nm in methanol at 0.0005%
800-1100 at 623-627 nm in methanol at 0.0005%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

General description

The dry mixture of the Leishman′s eosin methylene blue dye for microscopy, is used for the self-preparation of the Leishman′s staining solution. The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue and May-Grünwald′s eosin methylene blue, it is used for staining of blood and bone marrow smears and clinical cytological specimens. The dye is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Identity (UV/VIS-Spectrum): passes test
pH-value (0,01 %; methanol 50 %): 5 - 6
Absorption maximum λ 1 (Methanol): 623 - 627 nm
Absorption maximum λ 2 (Methanol): 522 - 525 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.0005 %; methanol; calculated on dried substance:
800 - 1100
- λ2max; 0.0005 %; methanol; calculated on dried substance: 650 - 850
TLC-Test: passes test
Loss on drying (105 °C): ≤ 8 %
Suitability for microscopy: passes test

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 - H317 - H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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