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HPLC Analysis of Withania Compounds on Ascentis® Express Phenyl-Hexyl

HPLC Analysis of Withania Compounds on Ascentis® Express Phenyl-Hexyl application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53336-U

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

94186

Withanoside IV

analytical standard

94187

12-Deoxywithastramonolide

analytical standard

74776

Withanolide A

analytical standard

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

mobile phase

[A] water; [B] acetonitrile

gradient

20 to 100% B in 10 min; held at 100% B for 0.5 min

flow rate

0.3 mL/min

pressure

2321 psi (160 bar)

column temp.

35 °C

detector

UV, 227 nm

injection

5 μL

sample

20 μg/mL in water: methanol (80:20)

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express Phenyl-Hexyl for the efficient separation of withania standards.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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