HPLC Analysis of Drugs of Abuse on Ascentis® Express F5
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
Benzoylecgonine solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Fentanyl solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(±)-Methamphetamine solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(+)-Propoxyphene solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase
[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water
gradient
held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min
flow rate
0.3 mL/min
pressure
175 bar
column temp.
35 °C
detector
ESI(+), m/z 100-1000
injection
1 μL
sample
1 μg/mL in 50:50, water: methanol
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany