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ENaC activity is regulated by calpain-2 proteolysis of MARCKS proteins.

American journal of physiology. Cell physiology (2017-05-05)
Darrice S Montgomery, Ling Yu, Zinah M Ghazi, Tiffany L Thai, Otor Al-Khalili, He-Ping Ma, Douglas C Eaton, Abdel A Alli
ABSTRACT

We previously demonstrated a role for the myristoylated alanine-rich C kinase substrate (MARCKS) to serve as an adaptor protein in the anionic phospholipid phosphate-dependent regulation of the epithelial sodium channel (ENaC). Both MARCKS and ENaC are regulated by proteolysis. Calpains are a family of ubiquitously expressed intracellular Ca

MATERIALS
Product Number
Brand
Product Description

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Calpain Inhibitor II, powder
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Monoclonal ANTI-FLAG® M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
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Calpastatin Peptide Ac 184-210, ≥95% (HPLC)
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Aldosterone, ≥95% (HPLC)