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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Toluene, anhydrous, 99.8%
Sigma-Aldrich
4-Methylmorpholine, purified by redistillation, ≥99.5%
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4-Methylmorpholine, purum, ≥98.0% (GC)
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4-Methylmorpholine, BioXtra, suitable for protein sequencing, ≥99.5% (GC)
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N,N-Diisopropylethylamine, purified by redistillation, 99.5%
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Pyridine, ≥99%
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(3-Glycidyloxypropyl)trimethoxysilane, ≥98%
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Methanol-12C, 99.95 atom % 12C
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4-Methylmorpholine, ReagentPlus®, 99%
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Calcium chloride, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
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Calcium chloride, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis
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N,N-Diisopropylethylamine, ReagentPlus®, ≥99%
Sigma-Aldrich
Calcium chloride, anhydrous, powder, 99.99% trace metals basis
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Triphenyl phosphate solution, NMR reference standard, 3 mM in chloroform-d (99.8 atom % D), NMR tube size 5 mm × 8 in.
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Methanol solution, (Methanol:Dimethyl sulfoxide 1:1 (v/v))
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Pyridine, Pharmaceutical Secondary Standard; Certified Reference Material
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Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
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Triphenyl phosphate solution, NMR reference standard, 0.0485 M in acetone-d6 (99.9 atom % D)
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Calcium chloride
Sigma-Aldrich
Triphenyl phosphate, ≥99%
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Calcium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Supelco
Residual Solvent - Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
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Pyridine, biotech. grade, ≥99.9%
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Pyridine, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5% (GC)
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N,N-Diisopropylethylamine, 99.5%, biotech. grade
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Pyridine, ACS reagent, ≥99.0%
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Pyridine, ReagentPlus®, ≥99%
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