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  • Effects of phthalate ester treatment on seed germination and antioxidant enzyme activities of Phaseolus radiatus L.

Effects of phthalate ester treatment on seed germination and antioxidant enzyme activities of Phaseolus radiatus L.

Bulletin of environmental contamination and toxicology (2014-02-19)
Wenli Liu, Chongbang Zhang, Shuyuan Liu
ABSTRACT

Effects of di-(2-ethylhexyl) phthalate and di-n-butyl phthalate on seed germination rate and antioxidant enzymes activities of mung bean (Phaseolus radiatus L.) were investigated. Results showed that under the treatment with 10 mg/kg of phthalate esters (PAEs), superoxide dismutase (SOD), peroxidase and catalase (CAT) activities were higher than those of the control (p > 0.05). But SOD and CAT activities decreased with the PAEs concentrations and the treatment duration, and were significantly lower than those of the control (p < 0.05). Effect of PAEs stress on SOD activity in germinating seeds of mung bean displayed a significant dose-effect relationship.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium hydrogen phthalate, BioXtra, ≥99.95%
Sigma-Aldrich
Phthalic acid, puriss. p.a., ≥99.5% (T)
Sigma-Aldrich
Phthalic acid, reagent grade, 98%
Sigma-Aldrich
Phthalic acid, ACS reagent, ≥99.5%
Supelco
Phthalic acid, analytical standard
Phthalic acid, European Pharmacopoeia (EP) Reference Standard
Supelco
Potassium phthalate monobasic, Standard for quantitative NMR, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Potassium phthalate monobasic, Vetec, reagent grade, 99%
Sigma-Aldrich
Potassium phthalate monobasic, BioUltra, ≥99.5% (T)
Sigma-Aldrich
Potassium phthalate monobasic, puriss. p.a., ≥99.5% (T)
Supelco
Potassium phthalate monobasic, reference material for titrimetry, certified by BAM, >99.5%
Sigma-Aldrich
Phthalic acid dipotassium salt, 98%
Sigma-Aldrich
Potassium phthalate monobasic, ACS reagent, acidimetric standard