Formation potential of emerging disinfection by-products during ozonation and chlorination of sewage effluents.

This study investigates the formation potential of emerging DBPs (haloacetonitriles, halonitromethanes and halopropanones) during ozonation and ozonation/hydrogen peroxide treatment and subsequent chlorination of sewage effluent under various experimental conditions. Estimation of possible risk due to DBPs by calculation of cytotoxicity and genotoxicity was attempted. The studied DBPs showed different formation behavior during chlorination, with maximum yields within 0.5-48 h. Maximum cytotoxicity and genotoxicity was observed after 4 h of chlorination with dibromoacetonitrile being the major contributor. Ozonation and O3/H2O2 treatment resulted in increase of trichloronitromethane followed by a decline at higher doses, and reduction of haloacetonitriles. High ozone doses reduced cytotoxicity and genotoxicity of treated effluents. The presence of bromide shifted to bromo-DBPs formation and enhanced both cytotoxicity and genotoxicity. Particulate fraction in effluents significantly contributed to the formation of DBPs and consequently to the their toxicity.