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Protein Gel Electrophoresis and Transfer Equipment

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Rapid gel casting with the mPAGE® Lux Casting System.
mPAGE® Electrophoresis Gel Caster
Auto2D® 2D-PAGE and 2D-DIGE Device

What is protein gel electrophoresis? Protein gel electrophoresis is a method that often uses various systems and transfer equipment to separate a diverse mixture of proteins by their molecular weight. Subsequent protein analyses include protein purification, proteomics, and Western Blotting applications. A variety of electrophoresis cells and transfer systems for polyacrylamide gel electrophoresis (PAGE), SDS-PAGE, 2D-PAGE, and wet protein transfer systems are available for your needs.

Explore our rapid gel casting, electrophoresis, and transfer equipment product solutions:

mPAGE® Lux Casting System for SDS-PAGE

Add mPAGE® Lux Casting System to your lab and have ready-to-go gels in under 3 minutes. Take a breath and get used to a fast gel casting process with less waste and without the worry of failure. That’s fresh.

The mPAGE® Lux Casting System replaces your SDS-PAGE gel casting process with faster, simpler, and safer methods with more reproducible results.

  • Cure a mini gel in 90 seconds with the mPAGE® Lux Casting System compared to conventional methods that take more than 90 minutes.
  • Bis-Tris gel chemistry allows for shorter run times compared to conventional methods.
  • mPAGE® Caster prevents leakage.
  • Superior band quality compared to Tris-Glycine gels.
  • All buffers and stains can be shared as the same chemistry is used with mPAGE® TurboMix casting kits and mPAGE® precast gels.
  • Less toxic chemicals used. No APS or TEMED required.
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Auto2D® 2D-PAGE and 2D-DIGE Device

Figure 1.Auto2D® 2D-PAGE and 2D-DIGE Device.

Auto2D® Electrophoresis Device

Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requiring advanced user training while offering low reproducibility and high inter-operator variability.

The Auto2D® 2-D Electrophoresis Device provides a fully automatable user-friendly solution for highly reproducible, rapid results and analysis.

  • Fully automated to eliminate tedious handling steps
  • Fast results in 1-2 hours
  • High reproducibility 
  • Easy to use, with no advanced training required
  • Lower inter-operator variability
  • Quick workflow implementation for reduced lab downtime
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mPAGE® GEL CASTER AND MINI GEL SYSTEM

Discover our new mPAGE® complete solution for casting mini gels. The mPAGE® Gel Caster and Mini Gel System offer a leak-free solution for hand-casting up to 2 mini protein gels, high-resolution protein separation on precast or hand-cast gels, and electrophoretic transfer to blotting membranes. For outstanding resolution and reduced run times, use our TurboMix® Bis-Tris Acrylamide Gel Casting and mPAGE® Bis-Tris Precast Gel solutions along with the compatible mPAGE® Buffer Systems.

mPAGE® GEL CASTER FEATURES AND BENEFITS

  • Simplify hand casting gels and eliminate leakage during the casting process
  • Hand cast up to 2 mini gels (8 x 10 cm) at one time
  • Maximum flexibility with gel caster available as a standalone device or comes as a device kit with the plates and combs
  • Mini Short Plates and Spacer Plates: 0.75 mm, 1.0 mm, or 1.5 mm thickness
  • Combs: 10 wells and 15 wells

mPAGE® MINI GEL SYSTEM FEATURES AND BENEFITS

  • Prevents incorrect assembly and leakage
  • Compatible with gels cast on the mPAGE® Gel Caster and mPAGE® Precast Gels
  • Available in 2 formats for running up to 2 or 4 mini gels (8 x 10 cm)
  • Accommodates 2 gel holder cassettes for protein transfer to Immobilon® PVDF or NC blotting membrane

Related Product Resources

Related Product Categories
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.
Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Transfer Buffers & Reagents
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
Related Applications
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Protein Expression
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Nucleic Acid Gel Electrophoresis
Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern and Southern blotting applications.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Protein Pull-Down
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.

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