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S5394

Sigma-Aldrich

Lipoprotein Deficient Serum from fetal calf

frozen liquid

Synonym(s):

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.61

biological source

bovine fetus

Quality Level

form

frozen liquid

concentration

≥14 mg protein/mL Biuret

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

impurities

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

shipped in

dry ice

storage temp.

−20°C

General description

Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.

Application

Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).

Preparation Note

Aseptically filtered, 0.2 μm
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Utsab Subedi et al.
International journal of molecular sciences, 24(3) (2023-02-12)
Neointimal hyperplasia is characterized by a loss of the contractile phenotype of vascular smooth muscle cells (VSMCs). Our group has recently shown that VSMC proliferation and migration are mediated by lysophosphatidic acid (LPA) during restenosis, but the role of autotaxin
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)

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