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MAK012

Sigma-Aldrich

Galactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Galactose Colorimetric/Fluorometric Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

pediatric diseases; neonatal diseases

storage temp.

−20°C

General description

The new Galactose Assay Kit, MAK502, is now available! Galactose is a simple monosaccharide that serves as an energy source and as an essential component of glycolipids and glycoproteins. Galactose contributes to energy metabolism via its conversion to glucose by the enzymes that constitute the Leloir pathway. Defects in the genes encoding these proteins lead to the metabolic disorder galactosemia.

Application

  • Galactose assay kit has been used in the determination of reactor galactose concentration.
  • It has been used in D-galactose assay using the galactose oxidase enzymatic microreactor.
  • It has also been used in the general synthesis and characterization of O-(2-aminoethyl)-O′-galactosyl polyethylene glycol (H2N-PEG-GAL) derivative.

Suitability

This kit is suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Principle

In this assay kit, Galactose is oxidized resulting in a colorimetric (570 nm)/fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the galactose present. This kit has a linear range of detection between 0.2-1.0 nmole galactose for the fluorometric assay and 2-10 nmole galactose for the colorimetric assay.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334,H412

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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isorders of Carbohydrate Metabolism
David R, et al.
Emery and Rimoin's Principles and Practice of Medical Genetics (2013)
Joana Silva et al.
Journal of materials chemistry. B, 10(26), 5016-5027 (2022-06-21)
Oxime formation is a convenient one-step method for ligating reducing sugars to surfaces, producing a mixture of closed ring α- and β-anomers along with open-chain (E)- and (Z)-isomers. Here we show that despite existing as a mixture of isomers, N-acetylglucosamine
Galactosylated polyaspartamide copolymers for siRNA targeted delivery to hepatocellular carcinoma cells
Gennara C, et al.
International Journal of Pharmaceutics, 525(2), 397-406 (2017)
A system identification approach for developing model predictive controllers of antibody quality attributes in cell culture processes
Brandon D, et al.
Biotechnol. Progress, 33(6) (2017)
Rapid and simple preparation of thiol?ene emulsion-templated monoliths and their application as enzymatic microreactors
Josiane P L, et al.
Royal Society of Chemistry, 15, 2162-2172 (2015)
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Protocols

Enzymatic Method for Determining Galactose (Galactose Assay)

This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose Assay Kit.

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