GE17-5181-01
SOURCE™ 15Q 4.6/100 PE
Synonym(s):
SOURCE 15Q Column
About This Item
Recommended Products
ligand
quaternary ammonium
material
polypropylene column
description
Ion Exchanger Type (value)
packaging
pkg of 1 ea
parameter
580 psi
bed size
4.6 mm × 100 mm
bed volume
1.7 mL
column I.D.
4.6 mm
matrix
polymeric
polystyrene/divinylbenzene
matrix active group
ammonium ion, quaternary phase
avg. part. size
15 μm
cleaning
1-14
working range
2-12
suitability
suitable for bioprocess medium
separation technique
anion exchange
Related Categories
General description
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Features and Benefits
- SOURCE™ 15Q media is highly suitable for fast, high-resolution purifications and easy scale-up
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocols
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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