HPLC Analysis of an Energy Drink on Ascentis^® Express HILIC (ELSD Detection)

column

Ascentis Express HILIC, 5 cm x 3 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5 (titrated with acetic acid) [B] water [C] acetonitrile; (9:1:90, A:B:C:)

flow rate

0.6 mL/min

column temp.

35 °C

detector

ELSD, 55° C, 3.5 bar nitrogen

injection

2 μL

sample

Energy Drink Diluted 1:9 in acetonitrile