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Key Documents

G001087

HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery RP-AmideC16, 25 cm × 4.6 mm I.D., 5 μm particles (505013)
mobile phase: [A] 0.1% TFA, 1% acetonitrile in water; [B] 0.1% TFA, 40% acetonitrile
gradient: 0-100% B in 130 min
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 215 nm

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Cytochrome c, species variants

Analytical column


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