53342-U

Ascentis^® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 5 cm × 3 mm, HPLC Column

• Synonym(s): Core-shell (SPP) Fused Core Phenyl HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® Express Phenyl-Hexyl, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 3 mm
• material: stainless steel column
• agency: suitable for USP L11
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 5 cm × 3 mm
• surface area: 135 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: phenylhexyl phase
• particle size: 2.7 μm
• pore size: 90 Å
• pH range: 2-9
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application

• A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers: Demonstrates the robustness of the Ascentis^® Express Phenyl-Hexyl, 2.7 μm HPLC column in automating the detection of contaminants in beverages. This study showcases the column′s capacity for high throughput analysis, essential for quality control in the food industry, ensuring safety and compliance with regulatory standards (Lhotská et al., 2016).


• Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids: This research highlights the efficiency of the Ascentis^® Express Phenyl-Hexyl column in analyzing complex phenolic compounds, illustrating its superior performance in environmental and pharmaceutical applications. The column′s design enables enhanced separation capabilities, crucial for accurate phenolic profiling in various samples (Chocholouš et al., 2013).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany